PNGase F from Elizabethkingia miricola
PNGase F from Elizabethkingia miricola
Application
Used to deglycosylate protein.
Biochem/physiol Actions
Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.
PNGase F from Elizabethkingia miricola
Unit Definition
One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.
Physical form
Solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA
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